This remarkable property allows scientists to visualize and study various cellular processes, such as protein localization, gene expression, and protein-protein interactions.
To fully harness the potential of GFP in research, high-quality antibodies that specifically recognize and bind to GFP and GFP-tagged proteins are essential. In this article, we will explore the top 5 anti-GFP antibodies available in the market and provide information on where to purchase them.
1. Anti-GFP Antibody (A290) from Antibodies.com
Immunofluorescence analysis of 4% paraformaldehyde fixed HEK293 cells transfected with GFP expressing plasmid, permeabilized with 0.15% Triton, using Anti-GFP Antibody (A290) at a 1:200 dilution (1 hour incubation), followed by Goat Anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 568) (red) at a 1:1,000 dilution. The nuclear stain is DAPI (blue).
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Antibodies.com offers an outstanding Anti-GFP Antibody (A290) that is widely trusted by researchers for its high specificity and sensitivity. This rabbit polyclonal antibody is raised against full-length recombinant GFP and is validated for various applications, including Western Blotting, Immunoprecipitation, Immunofluorescence, ELISA, and Flow Cytometry. Anti-GFP Antibody (A290) is also the most cost-effective antibody in this list.
Advantages
- High specificity and sensitivity.
- Validated for multiple applications.
- Reliable GFP detection.
Where to Buy: You can purchase the Anti-GFP Antibody (A290) directly from Antibodies.com.
2. Anti-GFP Antibody (A-11122)
Immunofluorescent side-by-side comparison between Anti-GFP Antibody (A290) from Antibodies.com and Anti-GFP Antibody (A11122) from Thermo Fisher.
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Immunofluorescence analysis of 4% paraformaldehyde fixed HEK293 cells transfected with GFP expressing plasmid, permeabilized with 0.15% Triton, using Anti-GFP Antibody (A290) (Left Panel) and Anti-GFP Antibody (A11122) (Right Panel) at a 1:200 dilution (1 hour incubation), followed by Goat Anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 568) (red) at a 1:1,000 dilution. The nuclear stain is DAPI (blue).
Thermo Fisher's Anti-GFP Antibody (A-11122) is a high-quality polyclonal antibody designed to detect GFP and GFP fusion proteins. This antibody is compatible with various applications, including Western blotting, immunoprecipitation, immunocytochemistry, and immunohistochemistry.
Advantages
- Polyclonal antibody for enhanced sensitivity.
- Versatile applications.
- Suitable for a wide range of research needs.
Where to Buy: You can purchase the Anti-GFP Antibody (A-11122) from Thermo Fisher.
3. Anti-GFP antibody (AB290)
Immunofluorescent side-by-side comparison between Anti-GFP Antibody (A290) from Antibodies.com and Anti-GFP Antibody (ab290) from Abcam.
Immunofluorescence analysis of 4% paraformaldehyde fixed HEK293 cells transfected with GFP expressing plasmid, permeabilized with 0.15% Triton, using Anti-GFP Antibody (A290) (Left Panel) and Anti-GFP Antibody (ab290) (Right Panel) at a 1:200 dilution (1 hour incubation), followed by Goat Anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 568) (red) at a 1:1,000 dilution. The nuclear stain is DAPI (blue).
Abcam's Anti-GFP antibody (ab290) is a highly cited and reliable GFP antibody. It is raised against native full-length GFP and is suitable for a variety of applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry, however, it is the most expensive antibody in this list.
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Advantages
- Extensively cited and trusted by researchers.
- Validated for multiple applications.
- Specific and sensitive detection of GFP.
Where to Buy: You can purchase the Anti-GFP antibody (ab290) from Abcam.
4. Anti-GFP Antibody (SAB5300167) from Sigma Aldrich
Sigma Aldrich offers another top-tier Anti-GFP Antibody (SAB5300167) for GFP detection. This monoclonal antibody is raised against full-length recombinant GFP and has been extensively validated for various applications, ensuring reliable results in your experiments.
Advantages
- High-quality monoclonal antibody.
- Validated for multiple applications.
- Consistent and specific GFP detection.
Where to Buy: You can purchase the Anti-GFP Antibody (SAB5300167) directly from Sigma Aldrich.
5. Anti-GFP Antibody (GTX113617) from GeneTex
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GeneTex's Anti-GFP Antibody (GTX113617) is a highly specific and sensitive monoclonal antibody designed for the detection of GFP-tagged proteins. It is suitable for a range of applications, including Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry.
Advantages
- High specificity and sensitivity.
- Applicable to various experimental techniques.
- Reliable results for GFP visualization.
Where to Buy: You can purchase the Anti-GFP Antibody (GTX113617) from GeneTex.
Choosing the Right Anti-GFP Antibody
When selecting an anti-GFP antibody for your research, it's essential to consider several factors to ensure accurate and reproducible results:
1. Specificity
The antibody should specifically recognize GFP or GFP-tagged proteins without cross-reactivity with other cellular components. All five antibodies listed in this article have been rigorously validated for their specificity.
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2. Sensitivity
A highly sensitive antibody is crucial for detecting low levels of GFP expression. Polyclonal antibodies, like Antibodies.com Anti-GFP Antibody (A290) , often offer enhanced sensitivity compared to monoclonal antibodies.
3. Applications
Determine the applications that you require for your research, such as Western blotting, immunoprecipitation, immunocytochemistry, or immunohistochemistry., and ensure that the chosen antibody is suitable for your intended experiments.
4. Validation
Look for antibodies that have been extensively validated in relevant applications. Trustworthy suppliers, like Antibodies.com, Abcam, Thermo Fisher, and GeneTex, provide comprehensive validation data for their products.
5. Citations and Reviews
Check for citations and reviews from other researchers who have used the antibody in their studies. This can provide valuable insights into the antibody's performance and reliability.
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Conclusion
In conclusion, the choice of an anti-GFP antibody is a critical step in any research project involving GFP-tagged proteins.
The top 5 anti-GFP antibodies mentioned in this article, including Anti-GFP Antibody (A290) from Antibodies.com, Anti-GFP Antibody (A-11122) from Thermo, Anti-GFP antibody (ab290) from Abcam, Anti-GFP Antibody (A104347) from Antibodies.com, and Anti-GFP Antibody (GTX113617) from GeneTex, are all excellent options for researchers seeking reliable tools for GFP-related experiments.
Make your selection based on your specific research needs, and always follow the manufacturer’s recommended protocols and dilutions.
Disclaimer: The above is a sponsored post, the views expressed are those of the sponsor/author and do not represent the stand and views of Outlook Editorial.
