On Thursday, The ruling Bharatiya Janata Party legislative council member Puttanna joined the Congress alleging large-scale corruption in the government.
Hardik Patel resigned from the Congress on Wednesday, months ahead of the Assembly polls in Gujarat.
Rio, a four-time chief minister, submitted his resignation to Governor La Ganesan at the Raj Bhavan.
As the producer, who is also the founder of the production company Friday Film House, disclosed the survivor's name and identity, which is an offence, another case was also filed against him.
Patnaik, who is all set to give a new look to his government before the 2024 elections to the Lok Sabha and assembly, had asked the ministers to resign.