Khawaja has struggled for form to begin the series and was left out of a squad of 12 named on Monday.
The left-hander made 122 runs at an average of 20.33 during the first three Tests, with Australia and England locked at 1-1 in the series.
With Steve Smith returning from concussion, the in-form Marnus Labuschagne appears set to bat at No. 3 when the Test begins at Old Trafford on Wednesday (September 5).
Pattinson has also been left out after taking five wickets at 33.40 in two appearances.
It leaves Pat Cummins, Peter Siddle, Mitchell Starc and Josh Hazlewood competing for three spots in the pace attack.
Starc is yet to play during the Ashes series but took match figures of 7-85 during a tour match against Derbyshire ahead of the fourth Test.
David Warner, Marcus Harris, Marnus Labuschagne, Steve Smith, Travis Head, Matt Wade, Tim Paine, Pat Cummins, Peter Siddle, Mitchell Starc, Nathan Lyon, Josh Hazlewood
For in-depth, objective and more importantly balanced journalism, Click here to subscribe to Outlook Magazine