"A 61-year-old male patient has died," Health Minister Fahrettin Koca said on Twitter, but giving no further details.
The confirmation of a second death comes after Koca said on Tuesday that an 89-year-old man was the first fatality in Turkey.
"The tests concluded today show the importance of following the measures which have been taken so far," he said.
Koca said there were 93 new cases, up from 98 and so bringing the total number of those recorded to 191.
President Recep Tayyip Erdogan earlier on Wednesday called on Turks to leave their homes as little as possible as he announced a USD 15 billion aid package to support the Turkish economy.
"If we apply the measures already outlined, we can keep the time at home limited to three weeks," Erdogan said in Ankara.
Turkey has already temporarily shut schools, universities and public spaces such as cinemas, theatres and gyms in a bid to contain the spread of the novel coronavirus. (AFP) AQS